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45-687 (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-687 (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

GOLGA6 Antibody

CATALOG NUMBER: 45-687

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DLPKEKADGTEQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 45kDa band in human liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.9kDa according to NP_001033729.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows localized cytoplasm staining consistent with Golgi. Recommended concentration, 3-6ug/ml.
Specificity:
GeneID 55889 may be a pseudogene, while GeneID 342096 is thought to encode a functional protein.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GOLGA6B
Additional Names:
GOLGA6B, GOLGA6, golgi autoantigen, golgin subfamily a, member 6, HGNC:13567, GLP, Golgi autoantigen, golgin subfamily a, 6, Golgin linked to PML, golgin-like protein, golgi autoantigen, golgin subfamily a, 6B, GOLGA, Golgin subfamily A member 6B
Protein Accession Number:
NP_061122.4,NP_001033729.1
Protein Gi Number:
94538359
Ncbi Gene Id Number:
55889;342096
Background Reference 1:
Gilles F, Goy A, Remache Y, Manova K, Zelenetz AD. Cloning and characterization of a Golgin-related gene from the large-scale polymorphism linked to the PML gene. Genomics. 2000 Dec 15;70(3):364-74.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-687

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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