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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Ramos cell line lysates (35ug/lane).
GUCY2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

GUCY2D Antibody

CATALOG NUMBER: 55-919

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
This GUCY2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-570 amino acids from the Central region of human GUCY2D.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
120 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GUCY2D
Additional Names:
Retinal guanylyl cyclase 1, RETGC-1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase, ROS-GC, GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1
Protein Accession Number:
Q02846
Protein Gi Number:
1345920
Ncbi Gene Id Number:
3000
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a retina-specific guanylate cyclase,which is a member of the membrane guanylyl cyclase family. Likeother membrane guanylyl cyclases, this enzyme has a hydrophobicamino-terminal signal sequence followed by a large extracellulardomain, a single membrane spanning domain, a kinase homologydomain, and a guanylyl cyclase catalytic domain. In contrast toother membrane guanylyl cyclases, this enzyme is not activated bynatriuretic peptides. Mutations in this gene result in Lebercongenital amaurosis and cone-rod dystrophy-6 diseases. [providedby RefSeq].
Background Reference 1:
Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)
Background Reference 2:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Background Reference 3:
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Background Reference 4:
Pasadhika, S., et al. Invest. Ophthalmol. Vis. Sci. 51(5):2608-2614(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-919

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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