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Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody . Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cell stained with HIP2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). HIP2 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis in 293,mouse C2C12 cell line lysates (35ug/lane).
Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody . Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

HIP2 Antibody

CATALOG NUMBER: 61-078

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This HIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-139 amino acids from the Central region of human HIP2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:100
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBE2K
Additional Names:
Ubiquitin-conjugating enzyme E2 K, Huntingtin-interacting protein 2, HIP-2, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2-25 kDa, Ubiquitin-conjugating enzyme E2(25K), Ubiquitin-conjugating enzyme E2-25K, Ubiquitin-protein ligase, UBE2K, HIP2, LIG
Protein Accession Number:
P61086
Protein Gi Number:
46577658
Ncbi Gene Id Number:
3093
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.
Background Reference 1:
Furukawa, Y., et al., Electrophoresis 21(2):338-346 (2000).
Background Reference 2:
Kikuchi, J., et al., Arterioscler. Thromb. Vasc. Biol. 20(1):128-134 (2000).
Background Reference 3:
Petersen, A., et al., Exp. Neurol. 157(1):1-18 (1999).
Background Reference 4:
Kalchman, M.A., et al., J. Biol. Chem. 271(32):19385-19394 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-078

List Size:
400 ul

List Price:

$495.00

Datasheet
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