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Western blot analysis of lysed extracts from HepG2 and 293 cells using Histone H2A.X (Ab-139).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H2A.X (Ab-139).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X (Ab-139).
Western blot analysis of lysed extracts from HepG2 and 293 cells using Histone H2A.X (Ab-139).

Histone H2A.X (Ab 139) Antibody

CATALOG NUMBER: 79-387

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Histone H2A.X (Ab-139) antibody was raised against a peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total Histone H2A.X protein.
Predicted Molecular Weight:
15 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
H2AFX
Additional Names:
H2AX, H2A.X, H2A/X, MCH6, H2AFX, H2a/x, HIST5-2AX
Protein Accession Number:
NP_002096.1
Protein Gi Number:
4504253
Ncbi Gene Id Number:
3014
Background:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
Background Reference 1:
Yaneva M, et al. (2005) Nucleic Acids Res. 33 (16): 5320-5330. Tsukuda T, et al. (2006) Nature. Author manuscript; available in PMC 2006 March 6

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-387

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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