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Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody.
Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody. The lane on the left is treated with antigen-specific peptide.

hnRPD (phospho Ser83) Antibody

CATALOG NUMBER: 79-815

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
hnRPD (phospho Ser83) antibody was raised against a peptide sequence around phosphorylation site of Serine 83 (N-S-S (p)-P-R) derived from Human hnRPD .
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
The antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.
Predicted Molecular Weight:
38 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HNRNPD
Additional Names:
ROD, AUF1, HNRPD
Protein Accession Number:
NP_112738.1
Protein Gi Number:
14110420
Ncbi Gene Id Number:
3184
Background:
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Background Reference 1:
Kajita Y., J. Biol. Chem. 270:22167-22175(1995).
Background Reference 2:
Dempsey L.A., Genomics 49:378-384(1998).
Background Reference 3:
Tolnay M., Biochem. J. 338:417-425(1999).
Background Reference 4:
Grosset C., Cell 103:29-40(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-815

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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