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Western blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of IL-22R in rat kidney tissue with IL-22R antibody at 5 μg/mL.
Immunofluorescence of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.
Immunocytochemistry of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.
Immunofluorescence of IL-22R in rat kidney tissue with IL-22R antibody at 20 μg/ml.
Western blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 μg/ml.
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IL-22 Receptor Antibody

CATALOG NUMBER: 2497

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%), Bovine: (88%)
Immunogen:
IL-22 receptor antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor.
The immunogen is located within the first 50 amino acids of IL-22 Receptor.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5-1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.               
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Predicted Molecular Weight:
Predicted: 63 kDa
Observed: 65 kDa
Purification:
IL-22 Receptor Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IL-22 Receptor Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-22 Receptor antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL22RA1
Additional Names:
IL-22 Receptor Antibody: IL22R, CRF2-9, IL22R1, IL22R, Interleukin-22 receptor subunit alpha-1, Cytokine receptor class-II member 9, IL-22 receptor subunit alpha-1
Protein Accession Number:
NP_067081
Protein Gi Number:
10719607
Ncbi Gene Id Number:
58985
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-22 Receptor Antibody: A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified. The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family. IL-22R forms a complex with IL-10 receptor beta chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-alpha 2 (IL-22RA2), was identified very recently. IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.
Background Reference 1:
Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9.
Background Reference 2:
Dumoutier L, Van Roost E, Colau D, Renauld JC.  Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. Proc Natl Acad Sci USA. 2000 ;97(18):10144-9.
Background Reference 3:
Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.
Background Reference 4:
Kotenko SV, Izotova LS, Mirochnitchenko OV, et al. Identification of the functional interleukin-22 (IL-22) receptor complex: J Biol Chem. 2001;276(4):2725-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2497

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$35 Dry Ice Shipment fee may be required on some items.

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