Many monoclonal antibodies work well in one application but not in others despite detecting the same proteins. The reason is because the target antigen may be denatured or the target epitope become inaccessible depending on the assay. For example in flow cytometry, the protein of interest is still in its native state while in western blotting the protein is typically denatured. Because of this nature of antibodies, scientists must search for and test multiple antibodies for one application and find that they need to repeat the process again for a different application. ProSci has recognized this issue and has released Immune Checkpoint Antibody Sets to provide a panel of antibodies against the same target so that researchers can find the optimal antibody for their experiment.
Each set consists of monoclonal antibodies developed with recombinant protein antigens expressed in mammalian cells to best recognize immune checkpoint targets in their native form. Rigorously tested in a variety of applications, the best antibodies were carefully selected and paired together to provide the broadest range of binding features for scientist to select from. Below are the available targets to choose from.
Developed with recombinant proteins expressed in mammalian cells to best recognize the native proteins.
Developed with recombinant proteins expressed in mammalian cells to best recognize the native proteins.
| Product Set | Clones | ELISA | WB | ICC | IF | IHC | Flow | Pairs |
|---|---|---|---|---|---|---|---|---|
| PD-1 | 5 | |||||||
| PD-L1 | 6 | |||||||
| PD-L2 | 5 | TBD | ||||||
| CTLA-4 | 3 | |||||||
| CD80 | 6 | TBD | ||||||
| TIGIT | 6 | |||||||
| LIGHT | 4 | |||||||
| VISTA | 4 | |||||||
| LAG-3 | 7 | |||||||
| B7-H3 | 5 | |||||||
| LAG-3 | 7 | |||||||
| TIM-3 | 7 | |||||||
| TMIGD2 | 3 |
Llama single domain antibodies (sdAbs) offer unique characteristics amenable to many applications. These sdAbs demonstrate low nanomolar binding affinity and can be used for the various applications.
