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Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 μg/mL..
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 μg/mL.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 μg/mL.
Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 μg/mL..
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IRF3 Antibody

CATALOG NUMBER: 3399

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3.
The immunogen is located within amino acids 150 - 200 of IRF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
IRF3 antibody can be used for detection of IRF3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Positive Control 3:
Cat. No. 1225 - Ramos Cell Lysate
Predicted Molecular Weight:
Predicted: 50 kDa
Observed: 50 kDa
Purification:
IRF3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IRF3 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRF3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRF3
Additional Names:
IRF3 Antibody: Interferon regulatory factor 3, IRF-3
Protein Accession Number:
NP_001562
Protein Gi Number:
4504725
Ncbi Gene Id Number:
3661
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRF3 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
Background Reference 1:
Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54.
Background Reference 2:
Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
Background Reference 3:
Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
Background Reference 4:
Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3399

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$35 Dry Ice Shipment fee may be required on some items.

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