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Western blot analysis of KLK6 using rabbit polyclonal KLK6 Antibody (L141) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KLK6 gene.
Kallikrein 6(KLK6) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Kallikrein 6(KLK6) Antibody for immunohistochemistry.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of KLK6 using rabbit polyclonal KLK6 Antibody (L141) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KLK6 gene.

Kallikrein 6 Antibody

CATALOG NUMBER: 62-247

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Kallikrein 6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-156 amino acids from the Central region of human Kallikrein 6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLK6
Additional Names:
Kallikrein-6, 3421-, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme, KLK6, PRSS18, PRSS9
Protein Accession Number:
Q92876
Protein Gi Number:
3914480
Ncbi Gene Id Number:
5653
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The KLK6 enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease.
Background Reference 1:
Christophi, G.P., et al., J. Neurochem. 91(6):1439-1449 (2004).
Background Reference 2:
Bayes, A., et al., Biol. Chem. 385(6):517-524 (2004).
Background Reference 3:
Pampalakis, G., et al., Biochem. Biophys. Res. Commun. 320(1):54-61 (2004).
Background Reference 4:
Ghosh, M.C., et al., Tumour Biol. 25(4):193-199 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-247

List Size:
400 ul

List Price:

$495.00

Datasheet
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