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Western Blot at 1:2000 dilution + TT whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Kallikrein 7(KLK7) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Kallikrein 7(KLK7) Antibody for immunohistochemistry.
Western Blot at 1:2000 dilution + TT whole cell lysate Lysates/proteins at 20 ug per lane.

Kallikrein 7 Antibody

CATALOG NUMBER: 62-248

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Kallikrein 7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-97 amino acids from the Central region of human Kallikrein 7.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLK7
Additional Names:
Kallikrein-7, hK7, Serine protease 6, Stratum corneum chymotryptic enzyme, hSCCE, KLK7, PRSS6, SCCE
Protein Accession Number:
P49862
Protein Gi Number:
1710878
Ncbi Gene Id Number:
5650
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The KLK7 enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis.
Background Reference 1:
Planque, C., et al., Biochem. Biophys. Res. Commun. 329(4):1260-1266 (2005).
Background Reference 2:
Ishida-Yamamoto, A., et al., J. Invest. Dermatol. 124(2):360-366 (2005).
Background Reference 3:
Vasilopoulos, Y., et al., J. Invest. Dermatol. 123(1):62-66 (2004).
Background Reference 4:
Santin, A.D., et al., Gynecol. Oncol. 94(2):283-288 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-248

List Size:
400 ul

List Price:

$495.00

Datasheet
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