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Western blot analysis of KappaB ras1 in RAW264.7 cell lysate with KappaB ras1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunocytochemistry of KappaB-ras1 in RAW264.7 cells with KappaB-ras1 antibody at 1 μg/mL.
Western blot analysis of KappaB ras1 in RAW264.7 cell lysate with KappaB ras1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Green Product
Trial Size

KappaB ras1 Antibody

CATALOG NUMBER: 2493

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
KappaB ras1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human KappaB ras1.
The immunogen is located within the last 50 amino acids of KappaB ras1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
KappaB ras1 antibody can be used for detection of KappaB ras1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1283 - RAW 264.7 Cell Lysate
Positive Control 2:
Cat. No. 17-203 - RAW 264.7 Cell Slide
Purification:
KappaB ras1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
KappaB ras1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
KappaB ras1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NKIRAS1
Additional Names:
KappaB ras1 Antibody: KBRAS1, kappaB-Ras1, KBRAS1, NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1
Protein Accession Number:
AAF34998
Protein Gi Number:
7008402
Ncbi Gene Id Number:
28512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KappaB ras1 Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody is specific for kappaB-ras1 and has no cross-reactivity to kappaB-ras2.
Background Reference 1:
Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
Background Reference 2:
Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem.2003; 278:23101-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2493

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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