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Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis in WiDr cell line lysates (35ug/lane).

MED30 Antibody

CATALOG NUMBER: 60-343

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MED30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-133 amino acids from the Central region of human MED30.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MED30
Additional Names:
Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30, TRAP/Mediator complex component TRAP25, Thyroid hormone receptor-associated protein 6, Thyroid hormone receptor-associated protein complex 25 kDa component, Trap25, MED30, THRAP6, TRAP25
Protein Accession Number:
Q96HR3
Protein Gi Number:
74731968
Ncbi Gene Id Number:
90390
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The multiprotein TRAP/Mediator complex facilitates geneexpression through a wide variety of transcriptional activators.MED30 is a component of this complex that appears to be metazoanspecific (Baek et al., 2002 [PubMed 11909976]).
Background Reference 1:
Rienzo, M., et al. Biochim. Biophys. Acta 1799(7):487-495(2010)
Background Reference 2:
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Background Reference 3:
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Background Reference 4:
Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-343

List Size:
400 ul

List Price:

$495.00

Datasheet
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