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MERS S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human DPPIV, Fc Tag at 5 ug/mL (100 uL/well) can bind MERS S protein RBD, His Tag with a linear range of 0.6-5 ng/mL (QC tested).
Immobilized MERS S protein RBD, His Tag at 5 ug/mL (100 uL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.313-2.5 ug/mL (Routinely tested).
MERS S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

MERS S Recombinant Protein RBD

CATALOG NUMBER: 11-178

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
MERS
source-species:
HEK293 cells
Recombinant Protein Sequence:
Glu 367 - Tyr 606
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
28.2 kDa
Purity:
>90% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Names:
Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,COVID-19
Background:
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Background Reference 1:
Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
Background Reference 2:
Benvenuto D, et al. J Med Virol. 2020.
Background Reference 3:
Chang CY, et al. AMB Express. 2020. 10(1):20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-178

List Size:
0.1 mg

List Price:

$658.00

Datasheet
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