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MGMT Recombinant Protein

CATALOG NUMBER: 92-212

Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Met1-Asn207
Fusion Tag:
N-6 His tag
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Predicted Molecular Weight:
23.8 kD
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
physical-state:
Liquid
Buffer:
Supplied as a 0.2 um filtered solution of 20mMTrisHCl,1mM EDTA, pH 8.0 . It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Storage Conditions:
Store at -20°C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Ncbi Official Symbol:
MGMT
Additional Names:
Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase, O-6-methylguanine-DNA-alkyltransferase, MGMT
Protein Accession Number:
P16455
Protein Gi Number:
530394832
Ncbi Gene Id Number:
4255
Background:
MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

92-212

List Size:
0.05 mg

List Price:

$515.00

Datasheet
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