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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Phospho-Ser189).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189).

MKK3 (phospho Ser189) Antibody

CATALOG NUMBER: 79-171

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
MKK3 (Phospho-Ser189) antibody was raised against a peptide sequence around phosphorylation site of serine 189 (V-D-S (p) -V-A) derived from Human MKK3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of MKK3 only when phosphorylated at serine189.
Predicted Molecular Weight:
40 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP2K3
Additional Names:
MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3
Protein Accession Number:
NP_002747.2
Protein Gi Number:
21618347
Ncbi Gene Id Number:
5606
Background:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Background Reference 1:
Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-171

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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