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Western blot analysis of MNDA Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in CEM cell line lysate
MNDA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MNDA Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in CEM cell line lysate

MNDA Antibody

CATALOG NUMBER: 55-731

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MNDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-53 amino acids from the N-terminal region of human MNDA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MNDA
Additional Names:
Myeloid cell nuclear differentiation antigen, MNDA
Protein Accession Number:
P41218
Protein Gi Number:
730038
Ncbi Gene Id Number:
4332
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The myeloid cell nuclear differentiation antigen (MNDA) isdetected only in nuclei of cells of the granulocyte-monocytelineage. A 200-amino acid region of human MNDA is strikinglysimilar to a region in the proteins encoded by a family ofinterferon-inducible mouse genes, designated Ifi-201, Ifi-202, andIfi-203, that are not regulated in a cell- or tissue-specificfashion. The 1.8-kb MNDA mRNA, which contains aninterferon-stimulated response element in the 5-prime untranslatedregion, was significantly upregulated in human monocytes exposed tointerferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS,CRP, and SPTA1. In its pattern of expression and/or regulation,MNDA resembles IFI16, suggesting that these genes participate inblood cell-specific responses to interferons.
Background Reference 1:
Kimkong, I., et al. J. Rheumatol. 37(7):1544-1547(2010)
Background Reference 2:
Kanellis, G., et al. Leukemia 23(10):1847-1857(2009)
Background Reference 3:
Joshi, A.D., et al. Clin. Cancer Res. 13 (18 PT 1), 5295-5304 (2007) :
Background Reference 4:
Briggs, R.C., et al. Cancer Res. 66(9):4645-4651(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-731

List Size:
400 ul

List Price:

$495.00

Datasheet
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