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42-967 (0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-967 (0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MYLK Antibody

CATALOG NUMBER: 42-967

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-KDTKNMEAKKLSKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 150kDa band observed in Human Colon and Lung lysates (calculated MW of 197kDa according to NP_444256.3). Recommended concentration: 0.01-0.03ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_444253.3; NP_444254.3 ; NP_444255.3 and NP_444256.3).
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2:
Cat. No. 1302 - Human Lung Tissue Lysate
Predicted Molecular Weight:
Approx 150 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MYLK
Additional Names:
MYLK, myosin, light chain kinase, DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK108, MLCK210, MSTP083, myosin light chain kinase, myosin, light polypeptide kinase
Protein Accession Number:
NP_444253.3,NP_444254.3,NP_444255.3,NP_444256.3
Protein Gi Number:
116008192
Ncbi Gene Id Number:
4638

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-967

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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