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43-395 (0.1ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-395 (0.1ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NBL1 Antibody

CATALOG NUMBER: 43-395

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KLGKTHGHRALE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in lysates of cell line Kelly (calculated MW of 23.2kDa according to NP_877421.2). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_877421.2) only.
Predicted Molecular Weight:
Approx 25kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NBL1
Additional Names:
NBL1, neuroblastoma, suppression of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3, DAN domain family member 1, differential screening-selected gene aberrant in neuroblastoma, neuroblastoma candidate region, suppression of tumorigenicity 1, neuroblastoma
Protein Accession Number:
NP_877421.2
Protein Gi Number:
115387106
Ncbi Gene Id Number:
4681
Background Reference 1:
Olakowski M, Tyszkiewicz T, Jarzab M, Król R, Oczko-Wojciechowska M, Kowalska M, Kowal M, Gala GM, Kajor M, Lange D, Chmielik E, Gubala E, Lampe P, Jarzab B.NBL1 and anillin (ANLN) genes over-expression in pancreatic carcinoma.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-395

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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