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Immunocytochemistry of NIK in Hek293 cells with NIK antibody at 10 μg/mL.
Immunofluorescence of NIK in Hek293 cells with NIK antibody at 20 μg/mL.<br><br>Green: NIK Antibody (1129) <br> Blue: DAPI staining
Immunocytochemistry of NIK in Hek293 cells with NIK antibody at 10 μg/mL.
Green Product
Trial Size

NIK Antibody

CATALOG NUMBER: 1129

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen:
NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK.
The immunogen is located within the last 50 amino acids of NIK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 104 kDa
Purification:
NIK Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
NIK Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
NIK antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP3K14
Additional Names:
NIK Antibody: HS, NIK, HSNIK, FTDCR1B, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, HsNIK
Protein Accession Number:
Q99558
Protein Gi Number:
92090612
Ncbi Gene Id Number:
9020
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NIK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
Background Reference 1:
Malinin NL, Boldin MP, Kovalenko AV, et al. MAP3K-related kinase involved in NF-κB induction by TNF, CD95 and IL-1. Nature 1997; 385:540-4.
Background Reference 2:
Regnier CH, Song HY, Gao X, et al. Identification and characterization of an IκB kinase. Cell 1997; 90:373-83.
Background Reference 3:
Woronicz JD, Gao X, Cao Z, et al. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997; 278:866-9.
Background Reference 4:
Ling L, Cao Z, and Goeddel D. NF-κB-inducing kinase activates IKK-α by phosphorylation of Ser-176. Proc. Natl. Acad. Sci. USA 1998; 95:3792-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

1129

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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