NKX2-8 Antibody

CATALOG NUMBER: 27-420

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NKX2-8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NKX2-8 antibody can be used for detection of NKX2-8 by ELISA at 1:12500. NKX2-8 antibody can be used for detection of NKX2-8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
26 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NKX2-8 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NKX2-8
Additional Names:
NKX2-8, NKX2.8, NKX2H, Nkx2-9
Protein Accession Number:
NP_055175
Protein Gi Number:
31377777
Ncbi Gene Id Number:
26257
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
A murine NKX2-8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. Nkx2.8 bound to the active AFP promoter, and antisense inhibition of Nkx2.8 mRNA translation selectively reduced expression of both the endogenous human AFP gene and transfected reporters containing the rat AFP promoter.
Background Reference 1:
Hamamoto, R., (2004) Nat. Cell Biol. 6 (8), 731-740.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-420

List Size:
100 ul

List Price:

$519.00

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