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46-881 (0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-881 (0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OTUB1 Antibody

CATALOG NUMBER: 46-881

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-YKEYAEDDNIYQQK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 30kDa band observed in Mouse Brain lysates (calculated MW of 31.3kDa according to Human NP_060140.2 and Mouse NP_598911.1). Recommended concentration: 0.03-0.1ug/ml.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
OTUB1
Additional Names:
OTUB1, OTU domain, ubiquitin aldehyde binding 1, FLJ20113, FLJ40710, HSPC263, MGC111158, MGC4584, OTB1, OTU1, OTU-domain Ubal-binding 1, otubain 1, ubiquitin-specific protease otubain 1
Protein Accession Number:
NP_060140.2
Protein Gi Number:
109148508
Ncbi Gene Id Number:
55611
Background Reference 1:
Juris SJ, Shah K, Shokat K, Dixon JE, Vacratsis PO. Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry. FEBS Lett. 2006 Jan 9;580(1):179-83.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-881

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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