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Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204).
Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204).

p44 / 42 MAP Kinase (phospho Tyr204) Antibody

CATALOG NUMBER: 79-215

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
p44/42 MAP Kinase (Phospho-Tyr204) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y (p) -V-A) derived from Human p44/42 MAP Kinase.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at tyrosine 204.
Predicted Molecular Weight:
42, 44 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAPK3
Additional Names:
ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, PKC2, ERK, Extracellular signal- regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1
Protein Accession Number:
NP_001035145.1
Protein Gi Number:
91718897
Ncbi Gene Id Number:
5595
Background:
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1.
Background Reference 1:
TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321Hironobu Ihn et al. (2000) Immunology 165: 2149-2155

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-215

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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