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Western blot analysis of lysed extracts from 293 cells untreated or treated with forskolin using PAK1 (Phospho-Thr212).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (Phospho-Thr212).
Western blot analysis of lysed extracts from 293 cells untreated or treated with forskolin using PAK1 (Phospho-Thr212).

PAK1 (phospho Thr212) Antibody

CATALOG NUMBER: 79-177

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PAK1 (Phospho-Thr212) antibody was raised against a peptide sequence around phosphorylation site of threonine 212 (P-V-T (p) -P-T) derived from Human PAK1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212.
Predicted Molecular Weight:
68 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PAK1
Additional Names:
PAKalpha, C1orf215, LAP18, OP18, p21-activated kinase 1, PAK-1, p65-PAK, Alpha-PAK
Protein Accession Number:
NP_001122092.1
Protein Gi Number:
190886457
Ncbi Gene Id Number:
5058
Background:
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Background Reference 1:
Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819Thiel DA, et al. (2002) Curr Biol; 12:1227-1232Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-177

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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