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Western blot analysis of PIAS1 in human kidney tissue lysate with PIAS1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PIAS1 in rat kidney tissue with PIAS1 antibody at 5 μg/mL.
Immunofluorescence of PIAS1 in rat kidney tissue with PIAS1 antibody at 20 μg/mL.
Western blot analysis of PIAS1 in human kidney tissue lysate with PIAS1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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PIAS1 Antibody

CATALOG NUMBER: 5741

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PIAS1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1.
The immunogen is located within the last 50 amino acids of PIAS1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PIAS1 antibody can be used for detection of PIAS1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
PIAS1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PIAS1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIAS1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIAS1
Additional Names:
PIAS1 Antibody: GBP, ZMIZ3, DDXBP1, GU/RH-II, E3 SUMO-protein ligase PIAS1, Gu-binding protein, GBP
Protein Accession Number:
NP_057250
Protein Gi Number:
7706637
Ncbi Gene Id Number:
8554
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIAS1 Antibody: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells.
Background Reference 1:
Liu B, Liao J, Rao X, et al. Inhibition of Stat1-mediated gene activation by PIAS1. Proc. Natl. Acad. Sci. USA1998; 95: 10626-31.
Background Reference 2:
Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol.2005; 5:593-605.
Background Reference 3:
Kang HC, Chae JH, Jeon J, et al. PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific {alpha}-globin expression. Nuc. Acids Res.2010 Apr 26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5741

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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