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Western blot analysis of extracts from mouse brain cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed A549 cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody.
Western blot analysis of extracts from mouse brain cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody. The lane on the right is treated with the antigen-specific peptide.

PKA alpha/beta CAT (phospho Thr197) Antibody

CATALOG NUMBER: 79-792

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p)-T-L) derived from Human PKA alpha/beta CAT.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.
Predicted Molecular Weight:
40 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PRKACA
Additional Names:
PRKACA , PRKACB, PKACA
Protein Accession Number:
NP_002721.1
Protein Gi Number:
4506055
Ncbi Gene Id Number:
5566
Background:
Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
Background Reference 1:
Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.
Background Reference 2:
W Li, Learn. Mem., Jan 1996; 2: 320 - 333.
Background Reference 3:
Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.
Background Reference 4:
Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-792

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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