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Western blot analysis of various lysates using α-Synuclein Rabbit mAb (A24951) at 1:7000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Negative control (NC): T-47D | Exposure time: 90s.
Confocal imaging of HEL cells using α-Synuclein Rabbit mAb (A24951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded human brain using α-Synuclein Rabbit mAb (A24951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded mouse brain using α-Synuclein Rabbit mAb (A24951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Immunoprecipitation of α-Synuclein in 600 µg extracts from Mouse brain using 3 µg α-Synuclein Rabbit mAb (A24951). Western blot analysis was performed using α-Synuclein Rabbit mAb (A24951) at 1:1000 dilution.
Western blot analysis of various lysates using α-Synuclein Rabbit mAb (A24951) at 1:7000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Negative control (NC): T-47D | Exposure time: 90s.

α-Synuclein Rabbit Recombinant Antibody

CATALOG NUMBER: 83-028

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IF/ICC, IP, WB
Application Note:
WB,1:3500 - 1:7000, IP,0.5μg-4μg antibody for 400μg-800μg extracts of whole cells, IF/ICC,1:200 - 1:800, IF-P,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
14kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SNCA
Additional Names:
PD1; NACP; PARK1; PARK4; α-Synuclein
Protein Accession Number:
P37840
Ncbi Gene Id Number:
6622
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-028

List Size:
100 uL

List Price:

$477.00

Datasheet
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