14-3-3 sigma Peptide

Application Note:

14-3-3 sigma Peptide is used for blocking the activity of 14-3-3 sigma antibody (catalog number 45-174). The 14-3-3 sigma antibody ships at 0.5 mg/mL and the 14-3-3 sigma Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.