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45-175 (0.03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-175 (0.03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

14-3-3 tau Antibody

CATALOG NUMBER: 45-175

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DDRKQTIDNSQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 28kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 27.8kDa according to NP_006817.1). Recommended concentration: 0.03-0.1ug/ml.
Positive Control 1:
Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
YWHAQ
Additional Names:
YWHAQ, 14-3-3 protein tau, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide, 14-3-3, 1C5, HS1, 14-3-3 protein T-cell, 14-3-3 protein theta, protein tau, tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide
Protein Accession Number:
NP_006817.1
Protein Gi Number:
5803227
Ncbi Gene Id Number:
10971
Background Reference 1:
Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M. Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle. Diabetes. 2007 Jun;56(6):1608-14. Epub 2007 Mar 16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-175

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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