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Western blot analysis of lysed extracts from HepG2 , HeLa and 293 cells using 14-3-3 zeta (Ab-58).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Ab-58).
Western blot analysis of lysed extracts from HepG2 , HeLa and 293 cells using 14-3-3 zeta (Ab-58).

14 3 3 zeta (Ab 58) Antibody

CATALOG NUMBER: 79-354

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
14-3-3 zeta (Ab-58) antibody was raised against a peptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total 14-3-3 zeta protein.
Predicted Molecular Weight:
28 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
YWHAZ
Additional Names:
HEL4, YWHAD, KCIP-1, HEL-S-3, 14-3-3-zeta, ERBB, ERBB1, HER1, 1433Z, 143Z, FAS, Factor activating exoenzyme S
Protein Accession Number:
NP_001129171.1
Protein Gi Number:
208973238
Ncbi Gene Id Number:
7534
Background:
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
Background Reference 1:
Gu YM, et al. (2006) FEBS Lett ; 580 (1): 305-310Powell DW, et al. (2003) Mol Cell Biol; 23 (15): 5376-5387Mackintosh C. (2004) Biochem J; 381 (Pt 2): 329-342.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-354

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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