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Western Blot in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of 27Kip1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western Blot in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.

27Kip1 Antibody

CATALOG NUMBER: 62-207

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Hamster
Immunogen:
This 27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the C-terminal region of human 27Kip1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDKN1B
Additional Names:
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
Protein Accession Number:
P46527
Ncbi Gene Id Number:
1027
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Background Reference 1:
Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005).
Background Reference 2:
Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005).
Background Reference 3:
Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005).
Background Reference 4:
Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-207

List Size:
400 ul

List Price:

$495.00

Datasheet
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