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Western blot analysis of lysed extracts from HeLa cells using 4E-BP1 (Phospho-Thr45).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45).
Western blot analysis of lysed extracts from HeLa cells using 4E-BP1 (Phospho-Thr45).

4E BP1 (phospho Thr45) Antibody

CATALOG NUMBER: 79-205

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
4E-BP1 (Phospho-Thr45) antibody was raised against a peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.
Predicted Molecular Weight:
18 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EIF4EBP1
Additional Names:
BP-1, 4EBP1, 4E-BP1, PHAS-I, STK14A, EIF4EBP1, PHAS-1
Protein Accession Number:
NP_004086.1
Protein Gi Number:
4758258
Ncbi Gene Id Number:
1978
Background:
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Background Reference 1:
Gingras AC, et al. (1998) Genes Dev 12 (4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23): 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5): 1087-1097. Moody CA, et al. (2005) J Virol 79 (9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4): 1432-1437.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-205

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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