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Western blot analysis of lysed extracts from C2C12 cells untreated or treated with Anisomycin using α-Synuclein (Phospho-Tyr125).
Immunofluorescence staining of methanol-fixed HeLa cells using α-Synuclein (Phospho-Tyr125).
Western blot analysis of lysed extracts from C2C12 cells untreated or treated with Anisomycin using α-Synuclein (Phospho-Tyr125).

a Synuclein (phospho Tyr125) Antibody

CATALOG NUMBER: 79-483

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
a-Synuclein (Phospho-Tyr125) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 125 (E-A-Y (p) -E-M) derived from Human α-Synuclein.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of α-Synuclein only when phosphorylated at tyrosine 125.
Predicted Molecular Weight:
18 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SNCA
Additional Names:
PD1, NACP, PARK1, PARK4, CD135, FLK2, STK1, SYN, SYUA, alpha-synuclein
Protein Accession Number:
NP_000336.1
Protein Gi Number:
4507109
Ncbi Gene Id Number:
6622
Background:
SncA is a member of the synuclein family of structurally related proteins that are prominently expressed in the central nervous system, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and SncA and Snc-Beta inhibit phospholipase D2 selectively. SncA may serve to integrate presynaptic signaling and membrane trafficking. Aggregated SncA proteins form brain lesions that are hallmarks of neurodegenerative synucleinopathies. Defects in SncA play a role in the pathogenesis of Parkinson disease. SncA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer disease. SncA shares 95% sequence homology with rat SncA. Rat SncA is specifically expressed in brain and is associated with synaptosomal membranes in neurons
Background Reference 1:
Takahashi T, et al. J Biol Chem 2003 Oct 24; 278 (43): 42225-33Ahn BH, et al. J Biol Chem 2002 Apr 05; 277 (14): 12334-42Negro A, et al. FASEB J 2002 Feb; 16 (2): 210-2Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-483

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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