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Western blot analysis of extracts of various cell lines, using AAMP antibody (19-018) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of 293T cells using AAMP antibody (19-018) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using AAMP antibody (19-018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

AAMP Antibody

CATALOG NUMBER: 19-018

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 85-434 of human AAMP (NP_001078.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:1000 - 1:3000
IF: 1:100 - 1:200
Positive Control 1:
A375
Positive Control 2:
HepG2
Positive Control 3:
293T
Positive Control 4:
MCF7
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse testis
Predicted Molecular Weight:
Observed: 47kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AAMP
Additional Names:
angio-associated migratory cell protein
Ncbi Gene Id Number:
14
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The gene is a member of the immunoglobulin superfamily. The encoded protein is associated with angiogenesis, with potential roles in endothelial tube formation and the migration of endothelial cells. It may also regulate smooth muscle cell migration via the RhoA pathway. The encoded protein can bind to heparin and may mediate heparin-sensitive cell adhesion.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-018

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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