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Western blot analysis of extracts of various cell lines, using AARS2 antibody (23-211) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded human liver damage using AARS2 antibody (23-211) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using AARS2 antibody (23-211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

AARS2 Antibody

CATALOG NUMBER: 23-211

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 841-985 of human AARS2 (NP_065796.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
SKOV3
Positive Control 3:
U-251MG
Positive Control 4:
MCF7
Predicted Molecular Weight:
Observed: 107kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AARS2
Additional Names:
AARSL, alanine tRNA ligase 2, mitochondrial (putative), alanine--tRNA ligase, alanyl-tRNA synthetase 2, alanyl-tRNA synthetase like, alanyl-tRNA synthetase, mitochondrial, alaRS, bA444E17.1, COXPD8, KIAA1270, MTALARS, MT-ALARS, OTTHUMP00000016528, probable alanyl-tRNA synthetase, AARS2 , AARSL
Ncbi Gene Id Number:
57505
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-211

List Size:
100 uL

List Price:

$436.00

Datasheet
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