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Western blot analysis in K562 cell line lysates (35ug/lane).
AARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

AARS2 Antibody

CATALOG NUMBER: 62-990

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-394 amino acids from the Central region of human AARS2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
107 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AARS2
Additional Names:
Alanine--tRNA ligase, mitochondrial {ECO:0000255, HAMAP-Rule:MF_03133}, 6117 {ECO:0000255, HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255, HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255, HAMAP-Rule:MF_03133}, AARS2 {ECO:0000255, HAMAP-Rule:MF_03133}
Protein Accession Number:
Q5JTZ9
Protein Gi Number:
74742244
Ncbi Gene Id Number:
57505
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNAAla <---> AMP + diphosphate + L-alanyl-tRNAAla.This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.
Background Reference 1:
Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)
Background Reference 2:
Nakayama,M., Genome Res. 12 (11), 1773-1784 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-990

List Size:
400 ul

List Price:

$495.00

Datasheet
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