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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

AASS Antibody

CATALOG NUMBER: 55-621

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AASS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 805-834 amino acids from the C-terminal region of human AASS.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
102 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AASS
Additional Names:
Alpha-aminoadipic semialdehyde synthase, mitochondrial, LKR/SDH, Lysine ketoglutarate reductase, LKR, LOR, Saccharopine dehydrogenase, SDH, AASS
Protein Accession Number:
Q9UDR5
Protein Gi Number:
46396032
Ncbi Gene Id Number:
10157
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a bifunctional enzyme that catalyzes thefirst two steps in the mammalian lysine degradation pathway. TheN-terminal and the C-terminal portions of this enzyme containlysine-ketoglutarate reductase and saccharopine dehydrogenaseactivity, respectively, resulting in the conversion of lysine toalpha-aminoadipic semialdehyde. Mutations in this gene areassociated with familial hyperlysinemia.
Background Reference 1:
Sacksteder, K.A., et al. Am. J. Hum. Genet. 66(6):1736-1743(2000)
Background Reference 2:
Papes, F., et al. Biochem. J. 344 PT 2, 555-563 (1999) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-621

List Size:
400 ul

List Price:

$495.00

Datasheet
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