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Antibody used in WB on Human Daudi at 5.0 ug/ml.
Antibody used in WB on Human Daudi at 5.0 ug/ml.

AATF Antibody

CATALOG NUMBER: 28-979

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AATF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
AATF antibody can be used for detection of AATF by ELISA at 1:312500. AATF antibody can be used for detection of AATF by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
63 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store AATF antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
AATF
Additional Names:
AATF, DED, BFR2, CHE1, CHE-1
Protein Accession Number:
NP_036270
Protein Gi Number:
7657013
Ncbi Gene Id Number:
26574
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The AATF is a protein that was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. AATF contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of AATF interfered with MAP3K12 induced apoptosis.The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.
Background Reference 1:
Lehner, B. (2004) Genome Res. 14 (7), 1315-1323.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-979

List Size:
100 ul

List Price:

$473.00

Datasheet
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