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ABCA12 Antibody

CATALOG NUMBER: 46-786

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: C-KDQKSYETADTSSQ
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Placenta, Skin and Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193kDa according to NP_775099.2 and of 257kDa according to NP_056472.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize both reported isoforms (NP_775099.2; NP_056472.2).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCA12
Additional Names:
ABCA12, ATP-binding cassette, sub-family A (ABC1), member 12, DKFZp434G232, FLJ41584, ICR2B, LI2, ATP-binding cassette, sub-family A, member 12
Protein Accession Number:
NP_775099.2,NP_056472.2
Protein Gi Number:
30795238
Ncbi Gene Id Number:
26154
Background Reference 1:
Sakai K, Akiyama M, Sugiyama-Nakagiri Y, McMillan JR, Sawamura D, Shimizu H Localization of ABCA12 from Golgi apparatus to lamellar granules in human upper epidermal keratinocytes Exp Dermatol. 2007 Nov;16(11):920-6

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-786

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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