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Western blot analysis of extracts of various cell lines, using ABCB8 antibody (18-750) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver damage using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ABCB8 Polyclonal Antibody (18-750) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ABCB8 antibody (18-750) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

ABCB8 Antibody

CATALOG NUMBER: 18-750

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 469-718 of human ABCB8 (NP_009119.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
Jurkat
Positive Control 3:
HepG2
Positive Control 4:
HeLa
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse skin
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ABCB8
Additional Names:
ABCB8, EST328128, M-ABC1, MABC1
Ncbi Gene Id Number:
11194
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-750

List Size:
100 uL

List Price:

$436.00

Datasheet
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