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Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
ABCC3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.

ABCC3 Antibody

CATALOG NUMBER: 55-057

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ABCC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-925 amino acids from the Central region of human ABCC3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
169 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ABCC3
Additional Names:
Canalicular multispecific organic anion transporter 2, ATP-binding cassette sub-family C member 3, Multi-specific organic anion transporter D, MOAT-D, Multidrug resistance-associated protein 3, ABCC3, CMOAT2, MLP2, MRP3
Protein Accession Number:
O15438
Ncbi Gene Id Number:
8714
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thesuperfamily of ATP-binding cassette (ABC) transporters. ABCproteins transport various molecules across extra- andintra-cellular membranes. ABC genes are divided into seven distinctsubfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Thisprotein is a member of the MRP subfamily which is involved inmulti-drug resistance. The specific function of this protein hasnot yet been determined; however, this protein may play a role inthe transport of biliary and intestinal excretion of organicanions. Alternatively spliced variants which encode differentprotein isoforms have been described; however, not all variantshave been fully characterized.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Hoffman, A.D., et al. Protein J. 29(5):373-379(2010)
Background Reference 3:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Background Reference 4:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-057

List Size:
400 ul

List Price:

$495.00

Datasheet
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