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Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HL-60 cell line lysates (35ug/lane).
ABCD1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

ABCD1 Antibody

CATALOG NUMBER: 55-187

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ABCD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-285 amino acids from the Central region of human ABCD1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ABCD1
Additional Names:
ATP-binding cassette sub-family D member 1, Adrenoleukodystrophy protein, ALDP, ABCD1, ALD
Protein Accession Number:
P33897
Protein Gi Number:
67476960
Ncbi Gene Id Number:
215
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ABCD1 is a member of thesuperfamily of ATP-binding cassette (ABC) transporters. ABCproteins transport various molecules across extra- andintra-cellular membranes. ABC genes are divided into seven distinctsubfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Thisprotein is a member of the ALD subfamily, which is involved inperoxisomal import of fatty acids and/or fatty acyl-CoAs in theorganelle. All known peroxisomal ABC transporters are halftransporters which require a partner half transporter molecule toform a functional homodimeric or heterodimeric transporter. Thisperoxisomal membrane protein is likely involved in the peroxisomaltransport or catabolism of very long chain fatty acids. Defects inthis gene have been identified as the underlying cause ofadrenoleukodystrophy, an X-chromosome recessively inheriteddemyelinating disorder of the nervous system.
Background Reference 1:
Matsukawa, T., et al. Neurogenetics (2010) In press :
Background Reference 2:
Xie, H.H., et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 27(2):144-148(2010)
Background Reference 3:
Li, J.Y., et al. J. Neurol. Sci. 290 (1-2), 163-165 (2010) :
Background Reference 4:
Hour, T.C., et al. Int. J. Biol. Markers 24(3):171-178(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-187

List Size:
400 ul

List Price:

$495.00

Datasheet
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