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46-796 (0.3μg/ml) staining of Jurkat lysate (35μg protein in RIPA buffer). Detected with chemiluminescence.
46-796 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-796 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-796 (0.3μg/ml) staining of Jurkat lysate (35μg protein in RIPA buffer). Detected with chemiluminescence.

ABCD4 Antibody

CATALOG NUMBER: 46-796

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RDDIDNPDQRISQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 68.6kDa according to NP_005041.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Kidney. Recommended concentration: 3.75ug/ml.
Specificity:
Reported variants represent identical protein: NP_064719.1 NP_064731.1 NP_064720.1 NP_064730.1
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCD4
Additional Names:
ABCD4, ATP-binding cassette, sub-family D (ALD), member 4, ABC41, EST352188, P70R, P79R, PMP69, PXMP1L, ATP-binding cassette, sub-family D, member 4, peroxisomal membrane protein 1-like
Protein Accession Number:
NP_005041.1
Protein Gi Number:
4826958
Ncbi Gene Id Number:
5826
Background Reference 1:
Asheuer M, Bieche I, Laurendeau I, Moser A, Hainque B, Vidaud M, Aubourg P. Decreased expression of ABCD4 and BG1 genes early in the pathogenesis of X-linked adrenoleukodystrophy. Hum Mol Genet. 2005 May 15;14(10):1293-303

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-796

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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