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45-183 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-183 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABCE1 Antibody

CATALOG NUMBER: 45-183

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KLNSIKDVEQKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 70kDa band observed in lysates of cell lines A431 (calculated MW of 67.6kDa according to NP_002931.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
Variants (NP_002931.2; NP_001035809.1) encode the same protein.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCE1
Additional Names:
ABCE1, RNase L inhibitor, RLI, OABP, ABC38, RNS4I, RNASEL1, RNASELI, ATP-binding cassette, sub-family E (OABP), member 1, ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) inhibitor, HP68, ATP-binding cassette, sub-family E, member 1
Protein Accession Number:
NP_002931.2,NP_001035809.1
Protein Gi Number:
4506559
Ncbi Gene Id Number:
6059
Background Reference 1:
Shichijo S, Ishihara Y, Azuma K, Komatsu N, Higashimoto N, Ito M, NakamuraT, Ueno T, Harada M, Itoh K. ABCE1, a member of ATP-binding cassette transporter gene, encodes peptides capable of inducing HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytesin colon cancer patients. Oncol Rep. 2005 May;13(5):907-13

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-183

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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