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43-566 (0.1ug/ml) staining of CACO-2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-566 (0.1ug/ml) staining of CACO-2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABCG2 / BCRP Antibody

CATALOG NUMBER: 43-566

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EPSKQDKPLIEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 70kDa band observed in lysates of cell line CACO-2 (calculated MW of 72.3kDa according to NP_004818.2). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
On restricted sale due to demands of patent holder. Not for sale in USA, if you are outside USA then please contact sales@everestbiotech.com to see if we can sell into your territory. This antibody is expected to recognize both reported isoforms (NP_004818.2; NP_001244315.1).
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCG2 / BCRP
Additional Names:
ABCG2, ATP-binding cassette, sub-family G (WHITE), member 2, ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR1, UAQTL1, ABC transporter, ATP-binding cassette sub-family G member 2, ATP-binding cassette transporter G2, breast
Protein Accession Number:
NP_004818.2,NP_001244315.1
Protein Gi Number:
62526033
Ncbi Gene Id Number:
9429
Background Reference 1:
Singh RR, Kunkalla K, Qu C, Schlette E, Neelapu SS, Samaniego F, Vega F.ABCG2 is a direct transcriptional target of hedgehog signaling and involved in stroma-induced drug tolerance in diffuse large B-cell lymphoma.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-566

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

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