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Western blot analysis of various lysates using ABflo® 488 Conjugated AIF1/IBA1 Rabbit mAb (A26032) at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20s.
Western blot analysis of lysates from Mouse spleen using ABflo® 488 Conjugated AIF1/IBA1 Rabbit mAb (A26032) at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.
Confocal imaging of paraffin-embedded Mouse brain tissue using ABflo® 488 Conjugated AIF1/IBA1 Rabbit mAb (A26032, dilution 1:500). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Human brain tissue using ABflo® 488 Conjugated AIF1/IBA1 Rabbit mAb (A26032, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat brain tissue using ABflo® 488 Conjugated AIF1/IBA1 Rabbit mAb (A26032, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of various lysates using ABflo® 488 Conjugated AIF1/IBA1 Rabbit mAb (A26032) at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20s.

ABflo® 488 Conjugated AIF1/IBA1 Rabbit Recombinant Antibody (83-205)

CATALOG NUMBER: 83-205

Clonality:
Monoclonal
Tested Applications:
IF-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
IF-P, WB
Application Note:
WB,1:2500 - 1:10000, IF-P,1:200 - 1:1000
Predicted Molecular Weight:
17kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze.
Ncbi Official Symbol:
Aif1
Additional Names:
G1; Iba1; AIF-1; D17H6S50E
Protein Accession Number:
O70200(mouse),P55008(Human)
Ncbi Gene Id Number:
11629
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables actin filament binding activity and calcium ion binding activity. Involved in several processes, including Rac protein signal transduction; actin filament organization; and ruffle assembly. Acts upstream of or within actin filament bundle assembly. Located in several cellular components, including actin filament; phagocytic cup; and ruffle membrane. Is expressed in adrenal cortex; central nervous system; embryo mesenchyme; and retina. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human AIF1 (allograft inflammatory factor 1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-205

List Size:
100 uL

List Price:

$640.00

Datasheet
Shipping Info

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