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Western blot analysis of various lysates using PSFlo 488 Conjugated TOM20 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.
Confocal imaging of HeLa cells using PSFlo 488 Conjugated TOM20 Rabbit mAb. DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Rat small intestine tissue using PSFlo 488 Conjugated TOM20 Rabbit mAb. DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of C2C12 cells using PSFlo 488 Conjugated TOM20 Rabbit mAb. DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of various lysates using PSFlo 488 Conjugated TOM20 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.

ABflo® 488 Conjugated TOM20 Rabbit Recombinant Antibody (83-331)

CATALOG NUMBER: 83-331

Clonality:
Monoclonal
Tested Applications:
IF-P, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
IF-P, IF/ICC, WB
Application Note:
WB,1:5000 - 1:30000, IF/ICC,1:200 - 1:1000, IF-P,1:200 - 1:1000
Predicted Molecular Weight:
16kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at 4℃. Avoid freeze.
Ncbi Official Symbol:
TOMM20
Additional Names:
MAS20; MOM19; TOM20
Protein Accession Number:
Q15388
Ncbi Gene Id Number:
9804
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables protein-transporting ATPase activity and unfolded protein binding activity. Involved in protein targeting to mitochondrion. Located in mitochondria-associated endoplasmic reticulum membrane and mitochondrial outer membrane.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-331

List Size:
100 uL

List Price:

$640.00

Datasheet
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