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Western blot analysis using ABL2 monoclonal antibody against truncated ABL2 recombinant protein.
Immunofluorescense analysis of ehtanol - fixed Hela cells showing cytoplasma staining using ABL2 antibody with IgG - FITC.
Western blot analysis using ABL2 monoclonal antibody against truncated ABL2 recombinant protein.

ABL2 Antibody [1H1B11]

CATALOG NUMBER: 32-104

Clonality:
Monoclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant ABL2 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Dilution
Western Blot:1:200 - 1:1,000
IF:1:100 - 1:500
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
ABL2 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ABL2
Additional Names:
ABLL, ARG, Abelson murine leukemia viral oncogene homolog 2ARG, ABLL
Protein Accession Number:
P42684
Protein Gi Number:
1168268
Ncbi Gene Id Number:
27
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ABL2(ARG, Abelson-related gene) is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. The ABL2 gene product is expressed as two variants bearing different amino termini, both approximately 12-kb in length. c-Abl shows both cytoplasmic and nuclear localizationc-Abl is involved in two different chromosomal translocations present in human leukemias, which generate Bcr-Abl and TEL-Abl.
Background Reference 1:
Yoshimi I, Takashi I, Tsuneyuki O, et al. Blood. 2000; 95(6): 2126-2131.
Background Reference 2:
Scheijen, B. and Griffin, J.D. Oncogene. 2002); 21 3314-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-104

List Size:
0.1 mL

List Price:

$408.00

Datasheet
Shipping Info

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