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45-188 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-188 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-188 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABTB1 Antibody

CATALOG NUMBER: 45-188

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: RALEDLLVSIGLDC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Human Heart lysates (calculated MW of 37.6kDa according to NP_115937.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABTB1
Additional Names:
ABTB1, BPOZ, EF1ABP, MGC20585, ankyrin repeat and BTB (POZ) domain containing 1, elongation factor 1A binding protein, Btb3, PP2259
Protein Accession Number:
NP_115937.1,NP_742024.1,NP_742025.1
Protein Gi Number:
14211841
Ncbi Gene Id Number:
80325
Background Reference 1:
Unoki M, Nakamura Y. Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway. Oncogene 2001 Jul 27;20(33):4457-65

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-188

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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