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45-189 (1.5ug/ml) as the reporter antibody.
45-189 (1.5ug/ml) as the reporter antibody.

ACACB Antibody

CATALOG NUMBER: 45-189

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QLGEPDLSDKDRKD
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277kDa according to NP_001084.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ACACB captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACACB
Additional Names:
ACACB, acetyl-Coenzyme A carboxylase beta , ACCB, HACC275 , acetyl-CoA carboxylase 2
Protein Accession Number:
NP_001084.2
Protein Gi Number:
61743950
Ncbi Gene Id Number:
32
Background Reference 1:
Reznick RM, Zong H, Li J, Morino K, Moore IK, Yu HJ, Liu ZX, Dong J, Mustard KJ, Hawley SA, Befroy D, Pypaert M, Hardie DG, Young LH, Shulman GI. Aging-Associated Reductions in AMP-Activated Protein Kinase Activity and Mitochondrial Biogenesis. Cell Metab. 2007 Feb;5(2):151-6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-189

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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