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45-190 staining (0.05ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-190 (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-190 (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-190 staining (0.05ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

ACADM Antibody

CATALOG NUMBER: 45-190

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RLIVAREHIDKYKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). Recommended for use at 0.01-0.1ug/ml. Primary incubation was for 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Adrenal Gland. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACADM
Additional Names:
ACADM, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, RP4-682C21.1, ACAD1, MCAD, MCADH, medium-chain acyl-CoA dehydrogenase, FLJ18227, FLJ93013, FLJ99884, medium-chain specific acyl-CoA dehydrogenase
Protein Accession Number:
NP_000007.1,NP_001120800.1
Protein Gi Number:
4557231
Ncbi Gene Id Number:
34
Background Reference 1:
Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K. Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1. Proc Natl Acad Sci USA. 1986 Sep;83(17):6543-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-190

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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